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. The peak wavelength is dependent on the collimator.
Thus, wavefront aberration, also called collimator error, is a deviation of
the collimated light from the intended or designed wavefront profile.
For edge-illuminated reflective imaging systems, this peak wavelength error
is inherently small. In the design of some commercial edge-illuminated
reflective imaging systems for microscopy, the wavefront
aberration was not considered. However, the
microscopic imaging performance, especially in the diffraction-limited
regime, can be improved significantly by careful
design (typically optimized in the micron range) of the optical
aperture and collimator.
For the specific case of the SIM device, which was optimized for Abbe
d’Espagnet’s design as well as several others’,
the values measured at a wavelength of 633 nm were about 80
nm. These values are sufficiently small that
diffraction is not a consideration at the resolution levels that are
generally achievable in practical microscopy. However, the aberration
has a significant effect on the numerical aperture at the
optical axis of the microscope and the FOV diffraction-limited
microscope resolution will be limited to about 60 nm.
One option is to optimize the system to minimize peak wavelength aberrations. A
fundamental (ideal) collimator is a device with a transmission function which
produces, when coupled to a conventional microscopic objective, diffraction-limited
microscope performance. No such device is generally available, however. (It
is not possible to derive a function that meets all the requirements of a
diffraction-limited microscope perfectly. What is needed are approximations
to a function that does well enough for a specific application.) One approach
is to invent approximations that do well, and to test these in the laboratory
with various techniques.
wavelength is the average of the wavefront aberration. In terms of the
structure factor, the mean wavelength is independent of collimator type, but
sensitive to the design of the aperture. The mean
wavelength is useful for estimating the numerical aperture of the
image. For example, for a Gaussian beam or a circular aperture, the numerical
aperture is.359, where is the mean wavelength in the
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